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Interferon gamma anticorps (Biotin)

Cet anticorps anti-Interferon gamma est un anticorps Souris Monoclonal détectant Interferon gamma dans ELISA, FACS et ELISpot. Adapté pour Boeuf (Vache), Mouton et Chévre. Ce Primary Antibody a été cité dans 4+ publications.
N° du produit ABIN6963775

Aperçu rapide pour Interferon gamma anticorps (Biotin) (ABIN6963775)

Antigène

Voir toutes Interferon gamma (IFNG) Anticorps
Interferon gamma (IFNG)

Reactivité

  • 255
  • 98
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  • 1
Boeuf (Vache), Mouton, Chévre

Hôte

  • 255
  • 185
  • 56
  • 12
  • 10
  • 4
  • 2
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Souris

Clonalité

  • 321
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  • 3
Monoclonal

Conjugué

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  • 90
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  • 1
  • 1
Cet anticorp Interferon gamma est conjugé à/à la Biotin

Application

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ELISA, Flow Cytometry (FACS), ELISpot

Clone

MT307
  • Specificité

    Native and recombinant IFN-γ

    Réactivité croisée (Details)

    The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.

    Attributs du produit

    This monoclonal antibody enables specific detection of bovine IFN-γ in immunoassays such as ELISpot, FluoroSpot, ELISA, and flow cytometry. Serum/Plasma samples

    Purification

    Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.,Purified from in vitro cultures by protein G affinity chromatography.

    Stérilité

    0.2 μm filtered

    Immunogène

    Recombinant bovine IFN-gamma

    Isotype

    IgG2a
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Commentaires

    ABIN6963775, ABIN7448244, ABIN6963776 and ABIN6963777 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb .1 . ABIN6963775, ABIN7448244, ABIN6963776 and ABIN6963777 is also recommended for use in flow cytometry.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

    Date de péremption

    18 months
  • Sajiki, Konnai, Okagawa, Nishimori, Maekawa, Goto, Ikebuchi, Nagata, Kawaji, Kagawa, Yamada, Kato, Nakajima, Suzuki, Murata, Mori, Ohashi: "Prostaglandin E2 Induction Suppresses the Th1 Immune Responses in Cattle with Johne's Disease." dans: Infection and immunity, Vol. 86, Issue 5, (2018) (PubMed).

    Orbegozo-Medina, Martínez-Sernández, González-Warleta, Castro-Hermida, Mezo, Ubeira: "Vaccination of sheep with Quil-A® adjuvant expands the antibody repertoire to the Fasciola MF6p/FhHDM-1 antigen and administered together impair the growth and antigen release of flukes." dans: Vaccine, Vol. 36, Issue 15, pp. 1949-1957, (2018) (PubMed).

    Rojas, Moreno, Valcárcel, Peña, Sevilla, Martín et al.: "Vaccination with recombinant adenoviruses expressing the peste des petits ruminants virus F or H proteins overcomes viral immunosuppression and induces protective immunity against PPRV challenge in ..." dans: PLoS ONE, Vol. 9, Issue 7, pp. e101226, (2015) (PubMed).

    Vordermeier, Dean, Rosenkrands, Agger, Andersen, Kaveh, Hewinson, Hogarth: "Adjuvants induce distinct immunological phenotypes in a bovine tuberculosis vaccine model." dans: Clinical and vaccine immunology : CVI, Vol. 16, Issue 10, pp. 1443-8, (2009) (PubMed).

  • Antigène

    Interferon gamma (IFNG)

    Autre désignation

    IFNG

    ID gène

    281237, 443396

    Pathways

    Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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